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Anti-cancer activity of Fluggea leucopyrus willd (Katupila) against human ovarian carcinoma and characterization of active compounds

Authors:

Vajira. P. Bulugahapitiya ,

University or Ruhuna, Matara, LK
About Vajira. P.
Department of Chemistry, Faculty of Science
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M. M. A. B. Munasinghe,

University or Ruhuna, Matara, LK
About M. M. A. B.
Department of Chemistry, Faculty of Science
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L. M. Hettihewa,

University or Ruhuna, Galle, LK
About L. M.
Department of Pharmacology, Faculty of Medicine
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N. Kihara

Kanagawa University, Hiratsuka, JP
About N.
Department of Chemistry, Faculty of Science
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Abstract

Fluggea leucopyrus Willd has been used in the treatment for cancers in the traditional system of medicine in Sri Lanka. This study was aimed on evaluation of anti-cancer activity of F. leucopyrus against Human Ovarian carcinoma and identification of active compounds. Anti-cancer activity of ethyl acetate (EtoAc) extract of leaves and two compounds isolated from EtoAc extract were evaluated using MTS and hTERT assays on Human Ovarian carcinoma (A 2780). MTS assay showed a significant anti-proliferation activity giving IC50 (concentration of extract/compound that inhibits 50% of the activity) values of 36.35±0.17μg/mL, 12.36±1.0 μg/mL and 48.53±1.43 μg/mL for EtoAc extract and two compounds (compound-1 & compound-2) respectively. A rapid depletion of hTERT content in Human Ovarian cancer cells was observed for the compound-2 with the concentration range 50-200 μg/mL. The compounds-1 was identified as bergenin, a known compound, whereas the compound-2 was identified as a diastereoisomer of bergenin (at C-9 and C-14,) with the help of NMR and Mass spectral data, and with the comparison of reported data. Brine Shrimp Lethality assay for EtoAc extract showed LC50 (Lethal concentration) values of 2779.63 μg/mL, implying that no toxicity of plant extract towards brine shrimp. As conclusion, anti-cancer activity of leaves of F. leucopyrus against Human Ovarian cancers is confirmed in this study, and bergenin and its isomer were identified as the responsible compounds for the anti-cancer activity of this plant.
DOI: https://doi.org/10.4038/jsc.v11i2.27
How to Cite: Bulugahapitiya, V.P., Munasinghe, M.M.A.B., Hettihewa, L.M. and Kihara, N., 2020. Anti-cancer activity of Fluggea leucopyrus willd (Katupila) against human ovarian carcinoma and characterization of active compounds. Journal of Science, 11(2), pp.12–26. DOI: http://doi.org/10.4038/jsc.v11i2.27
Published on 18 Dec 2020.
Peer Reviewed

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